{"created":"2023-05-15T13:45:09.570001+00:00","id":46,"links":{},"metadata":{"_buckets":{"deposit":"f69ee5b4-0161-48db-a4be-e357a04b5c7d"},"_deposit":{"created_by":4,"id":"46","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"46"},"status":"published"},"_oai":{"id":"oai:hirosakiuhw.repo.nii.ac.jp:00000046","sets":["5:6:10"]},"author_link":["287","293","295","286","289","294","291","296","292","297","288","290"],"item_3_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Effect of polymorphisms in the gamma-glutamyl carboxylase gene GGCX on gammacarboxylation of osteocalcin and bone mineral density: PCR-SSP–based genotyping"}]},"item_3_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"GGCX イデンシ タケイ ガ オステオカルシン ノ Glaカ ト コツミツド ニ オヨボス エイキョウ ダイイッポウ PCR-SSP ホウ ニヨル GGCX イデンシ タケイ ハンテイ シケン ノ ケントウ"}]},"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"39","bibliographicPageStart":"33","bibliographicVolumeNumber":"4","bibliographic_titles":[{}]}]},"item_3_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究の目的は、γ-グルタミルカルボキシラーゼ（GGCX）遺伝子多型が、オステオカルシンのGla化と骨密度に及ぼす影響を検討し、将来、骨代謝異常をきたす慢性疾患を持つ人々に骨量低下や骨粗鬆症の予防のための効果的な栄養指導を行うことである。その第１段階として、GGCX多型判定試験の判別法を確立する必要がある。今回、GGCX遺伝子多型判定試験は、PCR-SSP法（Polymerase Chain Reaction-Sequence Specific Primer Method）で行い、2 種類のリバースプライマー（8RAと8RG）を使用した。PCR反応条件を検討した結果、Sample 1 は8RAと8RG、Sample 2 は8RA、Sample 3 は8RGのプライマーによるバンドが検出された。またシークエンス解析の結果、標的とするGG型、AA型、GA型の塩基配列が確認できた。以上のことから、本方法はGGCX遺伝子多型判定試験の判別法として有効であることが証明された。","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医療福祉大学紀要, 4(1), 2013, p.33-39","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ハセガワ, ヒデタカ"}]},{"nameIdentifiers":[{}],"names":[{"name":"カミグチ, ヒロシ"}]},{"nameIdentifiers":[{}],"names":[{"name":"ムラカミ, ダイスケ"}]},{"nameIdentifiers":[{}],"names":[{"name":"マツキ, ヒデアキ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"Hasegawa, Hidetaka"}]},{"nameIdentifiers":[{}],"names":[{"name":"Kamiguchi, Hiroshi"}]},{"nameIdentifiers":[{}],"names":[{"name":"Murakami, Daisuke"}]},{"nameIdentifiers":[{}],"names":[{"name":"Matsuki, Hideaki"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前医療福祉大学紀要編集委員会"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12475995","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2185-0550","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"基礎医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"弘前医療福祉大学保健学部看護学科"},{"subitem_text_value":"東海大学教育・研究支援センター分子科学部門"},{"subitem_text_value":"弘前医療福祉大学保健学部看護学科"},{"subitem_text_value":"東海大学健康学部看護学科"},{"subitem_text_value":"Hirosaki University of Health and Welfare"},{"subitem_text_value":"Tokai University Education and Research Support Center"},{"subitem_text_value":"Hirosaki University of Health and Welfare"},{"subitem_text_value":"Tokai University School of Health Sciences"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長谷川, 秀隆"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"神口, 浩"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 大介"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松木, 秀明"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-15"}],"displaytype":"detail","filename":"HirosakiIryoFukushi_4(1)_33.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiIryoFukushi_4(1)_33.pdf","url":"https://hirosakiuhw.repo.nii.ac.jp/record/46/files/HirosakiIryoFukushi_4(1)_33.pdf"},"version_id":"e9105122-bfe0-4fb0-acde-bf95fb2ff255"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GGCX遺伝子多型","subitem_subject_scheme":"Other"},{"subitem_subject":"低カルボキシル化オステオカルシン","subitem_subject_scheme":"Other"},{"subitem_subject":"ビタミンK","subitem_subject_scheme":"Other"},{"subitem_subject":"骨密度","subitem_subject_scheme":"Other"},{"subitem_subject":"GGCX genotype testing","subitem_subject_scheme":"Other"},{"subitem_subject":"undercarboxylated osteocalcin","subitem_subject_scheme":"Other"},{"subitem_subject":"Vitamin K","subitem_subject_scheme":"Other"},{"subitem_subject":"bone mineral density","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"GGCX 遺伝子多型がオステオカルシンのGla化と骨密度に及ぼす影響 : 第一報　PCR-SSP 法によるGGCX 遺伝子多型判定試験の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"GGCX 遺伝子多型がオステオカルシンのGla化と骨密度に及ぼす影響 : 第一報　PCR-SSP 法によるGGCX 遺伝子多型判定試験の検討"}]},"item_type_id":"3","owner":"4","path":["10"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-14"},"publish_date":"2013-06-14","publish_status":"0","recid":"46","relation_version_is_last":true,"title":["GGCX 遺伝子多型がオステオカルシンのGla化と骨密度に及ぼす影響 : 第一報　PCR-SSP 法によるGGCX 遺伝子多型判定試験の検討"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-05-15T14:24:40.362452+00:00"}